基于UPLC-Q-TOF-MS的不同月份铁皮石斛次生代谢物动态变化规律研究

Study on Dynamic Variation Patterns of Secondary Metabolites in Dendrobium Officinale of Different Months Based on UPLC-Q-TOF-MS

摘要:
目的探究铁皮石斛中次生代谢产物在不同月份的变化规律，为铁皮石斛资源的精细化开发提供依据。
方法利用UPLC-Q-TOF-MS结合分子网络技术快速鉴定铁皮石斛中的次生代谢产物，并对连续12个月的铁皮石斛样品中酚类、黄酮等化合物的相对含量进行比较分析。
结果共鉴定出9类56种次生代谢产物，其中酚类、黄酮及萜类的相对含量均在11月达到最高。
结论 11月可作为铁皮石斛的最佳采收期，该研究有助于铁皮石斛资源的精准利用。

Abstract:
OBJECTIVE To explore dynamic variation patterns of secondary metabolites in Dendrobium officinale at different months, and to provide data for the precision utilization of Dendrobium officinale resources.
METHODS Secondary metabolites of Dendrobium officinale were rapidly identified using UPLC-Q-TOF-MS combined with molecular networking, and the relative contents of phenols, flavonoids, and other compounds of Dendrobium officinale for 12 consecutive months were analyzed.
RESULTS A total of 9 categories and 56 types of secondary metabolites were identified, and phenols, flavones, and terpenoids reached highest relative contents in November.
CONCLUSION November can be regarded as the potential optimal harvesting time for Dendrobium officinale, and this study is conducive to maximizing the utilization of Dendrobium officinale resources.