六神曲成分的快速鉴定及脂类活性研究

张希; 朱月健; 郑威; 宋孟霜; 栗嘉淇; 孔兵; 许枬

doi:10.13748/j.cnki.issn1007-7693.20232227

六神曲成分的快速鉴定及脂类活性研究

Rapid Identification and Lipid Activity Study of Constituents from the Massa Medicata Fermentata

摘要

摘要:
目的快速鉴定六神曲的成分，并探索主要成分及其活性。
方法采用HPLC-Q-TOF-MS与全球天然产物社交分子网络(global natural products social molecular networking，GNPS)分析结合的技术，通过Lipidomics、HMBD、METLIN等数据库匹配，依据文献数据、质谱裂解规律并结合GNPS分子网络分析，鉴定六神曲的化学成分，用细胞炎症试验评价六神曲总脂的抗炎作用。
结果从六神曲中共鉴定出72个化学成分，包括黄酮、倍半萜、鞘脂、甘油糖酯及取代苯类成分，总脂占比较大，且具有抗炎活性。
结论从六神曲中发现的磷脂酰胆碱、磷脂酰乙醇胺、双半乳糖甘油酯、单萜等10类成分未报道过，鉴定的72个成分中除化合物8、9、11~15、21、22外，其余63个成分为首次从六神曲中鉴定，为六神曲的发酵机制与活性成分研究提供了基础数据。

Abstract:
OBJECTIVE To quickly identify the components of Massa Medicata Fermentata(MMF) and explore the main ingredients and their activities.
METHODS Using HPLC-Q-TOF-MS and global natural products social molecular networking(GNPS) analysis, the chemical composition of MMF was identified by matching databases such as Lipidomics, HMBD, METLIN and so on. Based on literature data, mass spectrometry fragmentation patterns, and GNPS molecular network analysis, the anti-inflammatory effect of MMF total lipids was evaluated using cellular inflammation experiments.
RESULTS A total of 72 chemical components were identified from MMF, including flavonoids, sesquiterpenes, sphingolipids, glycerols, and substituted benzenes. The total lipids accounted for a large proportion and exhibited anti-inflammatory activity.
CONCLUSION The 10 types of components found in MMF, including phosphatidylcholine, phosphatidylethanolamine, galactose glycerides, and monoterpenes, have not been reported before, of the 72 components identified, 63 were identified from MMF for the first time except compound 8, 9, 11−15, 21, 22, which provides basic data for the study of the fermentation mechanism and active components of MMF.

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