辐照灭菌对覆盆子品质的影响

蔡子谦; 张文婷; 董婷; 程新梅; 杭丽凤; 丁盛; 郭增喜; 方翠芬; 陈碧莲

doi:10.13748/j.cnki.issn1007-7693.20230914

辐照灭菌对覆盆子品质的影响

Effect of Irradiation Sterilization on the Quality of Rubus Chingii Hu

摘要

摘要:
目的研究⁶⁰Co-γ辐照对覆盆子灭菌效果及主要成分的影响。
方法覆盆子经0、6、10、15、30　kGy剂量⁶⁰Co-γ辐照后，采用微生物计数法测定辐照前后覆盆子微生物水平；采用高分辨质谱法对覆盆子成分进行分析，对辐照前后覆盆子样品进行成分比较分析，鞣花酸及山柰酚3-O-芸香糖苷定量结果分析，指纹图谱相似度评价3个方面，考察辐照对覆盆子成分的影响。
结果不同剂量辐照处理后覆盆子微生物检查结果均符合要求；覆盆子辐照前后20个主要成分聚类分析和主成分分析提示差异无统计学意义；鞣花酸及山柰酚3-O-芸香糖苷含量差异均无统计学意义；辐照前后指纹图谱相似度均在0.995~1.000。
结论 ⁶⁰Co-γ辐照可有效控制覆盆子微生物水平，对覆盆子主要成分无显著影响，研究结果为辐照在覆盆子灭菌工艺中的应用提供依据。

Abstract:
OBJECTIVE To study the effect of ⁶⁰Co-γ irradiation on the sterilization effect and main components of Rubus chingii Hu.
METHODS Irradiated Rubus chingii Hu by 0, 6, 10, 15, 30 kGy doses of ⁶⁰Co-γ, used the microbial count method to determine the microbial level of Rubus chingii Hu before and after irradiation. Analyzed the components of Rubus chingii Hu by high resolution mass spectrometry, investigated the effects of irradiation on the quality of Rubus chingii Hu by comparing the components of Rubus chingii Hu samples before and after irradiation, analyzing the quantitative results of ellagic acid and kaempferol 3-O-yunxiangoside, and evaluating the similarity of fingerprints.
RESULTS The results of microbial examination of Rubus chingii Hu after different doses of irradiation all met the requirements, cluster analysis and principal component analysis of 20 components showed no significant difference. And there was no significant difference in the contents of ellagic acid and kaempferol 3-O-glucoside before and after irradiation. The similarity of fingerprints before and after irradiation was between 0.995 and 1.000.
CONCLUSION Irradiation can effectively control the microbial level in Rubus chingii Hu, and there is no significant effect on the chemical composition of Rubus chingii Hu, the results provide a basis for the application of irradiation in the sterilization process of Rubus chingii Hu.

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