Abstract: OBJECTIVE To investigate the anti-motion sickness efficacy of combination of cinnarizine tablets and methylphenidate. METHODS Changes in the electronystagmogram parameters of 8 male volunteers were recorded after rotatory stimulation. Twenty Beagle dogs were used in the animal experiment to induce salivation and vomiting symptoms by using a compound rotational stimulation pattern, and the latency of salivation and vomiting in each group of animals was compared. RESULTS The subjects took cinnarizine tablets or methylphenidate alone, or took cinnarizine tablets or methylphenidate in combination, which could inhibit the duration of rotational stimulation-induced nystagmus and the average slow-phase velocity at 1, 2, and 4 h after medication, and the efficacy of combined use of cinnarizine table and methylphenidate was stronger than that of cinnarizine table or methylphenidate alone. After the beagle dogs were treated with drugs, the latency of salivation and vomiting in the positive control drug diphenhydramine group and the low-dose group of narizine combined with methylphenidate was longer than that in the control group, but without statistical significance compared with the control group. However, the salivation and vomiting latencies treated with a medium-dose or a high-dose of combination of cinnarizine table and methylphenidate were significantly longer than control group, and the salivation and vomiting latencies of the medium-dose group were significantly longer than dimenhydrinate group. CONCLUSION The combination of cinnarizine tablets and methylphenidate reveals a significant anti-motion sickness effect, which maybe better than that of dimenhydrinate tablets.