Abstract: OBJECTIVE To analyze the main active components of Rehmanniae Radix against Alzheimer’s disease(AD) and its mechanism by using network pharmacology combined rat pheochromocytoma cells(PC12). METHODS Literature was reviewed to collect the chemical components of Rehmanniae Radix, the main active components were screened by TCMSP. And constructed the interaction network diagram of “Rehmanniae Radix-active components-potential targets” and protein-protein interaction network diagram. GO biological process enrichment and KEGG metabolic pathway enrichment were analyzed by DAVID database. Finally, MTT method was used to verify the protective effect of active ingredients on PC12 cells. RESULTS Network pharmacological analysis predicted fifteen main active ingredients of Rehmanniae Radix, and 343 targets associated with AD, which involved MAPK signaling pathway, metabolic pathways, insulin signaling pathway, PI3K-Akt signaling pathway, etc. The results of PC12 cells experiments showed that all the nine active ingredients of Rehmanniae Radix obtained by network pharmacological screening could improve the survival rate of PC12 cells to different degrees, among which the survival rate of PC12 cells treated with daucosterol, luteolin, Genipin-1-b-D-gentiobioside, apigenin, sucrose and raffinose was higher(P<0.05). CONCLUSION Rehmanniae Radix has a multi-component, multi-target and multi-pathway regulatory mechanism on AD, which provides reference for the intervention and treatment of AD by Rehmanniae Radix.