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    利用UHPLC-MS/MS靶标代谢组学研究柴芍皂苷对经前烦躁障碍症肝气郁证模型大鼠海马脑区神经递质的影响

    Effect of Saikosaponins and Paeoniflorin on Neurotransmitters in the Hippocampal Brain Region of Rats with Premenstrual Dysphoric Disorder Model of Liver Qi Depression Using UHPLC-MS/MS Target Metabolomics

      摘要
    • 摘要:
      目的  研究柴芍皂苷对经前烦躁障碍症(premenstrual dysphoric disorder，PMDD)肝气郁证模型大鼠的治疗作用及其对海马脑区神经递质的影响。
      方法 通过择时慢性束缚应激法制备PMDD肝气郁证大鼠模型，柴芍皂苷干预，旷场试验和强迫游泳试验评价大鼠抑郁症状，运用UHPLC-MS/MS靶标代谢组学检测大鼠海马脑区神经递质水平变化。
      结果 柴芍皂苷明显改善PMDD肝气郁证大鼠的抑郁症状。PMDD模型大鼠海马脑区5-HT、Glu、GABA、NE水平均显著升高，柴芍皂苷干预后5-HT、Glu、GABA、NE、DA和E水平与正常组比较差异均无统计学意义。
      结论 柴芍皂苷通过调节神经递质水平缓解PMDD肝气郁证大鼠抑郁症状，这可能是柴胡、白芍类方剂临床治疗PMDD肝气郁证的作用途径。

       

      Abstract:
      OBJECTIVE To study the therapeutic effect of saikosaponins and paeoniflorin on rats with premenstrual dysphoric disorder(PMDD) model of liver Qi depression and the effect on neurotransmitters in hippocampal brain area.
      METHODS The PMDD rat model of liver Qi depression was prepared by the time-chosen chronic restraint stress method, and the depressive symptoms of rats were evaluated by the intervention of saikosaponins and paeoniflorin, the open field experiment and forced swimming experiment, and the changes of neurotransmitter levels in the hippocampal brain region of rats were detected by UHPLC-MS/MS target metabolomics.
      RESULTS The levels of 5-HT, Glu, GABA and NE in the hippocampal brain area of PMDD rats were significantly increased, and the levels of 5-HT, Glu, GABA, NE, DA and E were not statistically significant from those of the normal group after the intervention of saikosaponins and paeoniflorin.
      CONCLUSION Saikosaponins and paeoniflorin alleviates depression in rats with PMDD by regulating neurotransmitter levels, and it may be the pathway for the clinical treatment of PMDD with liver qi depression in the formulations of Bupleuri Radix and Paeoniae Radix Alba.

       

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