基于UPLC-Q-TOF-MS技术快速分析樟帮九蒸九晒地黄的化学成分及固体辅料缓解地黄“滋腻碍脾”的功能研究

朱徐东; 姚伟; 严斐霞; 黄东; 王松; 朱娜; 袁铭铭; 黄丽萍; 吴毅

doi:10.13748/j.cnki.issn1007-7693.20223933

基于UPLC-Q-TOF-MS技术快速分析樟帮九蒸九晒地黄的化学成分及固体辅料缓解地黄“滋腻碍脾”的功能研究

Rapid Analysis of the Chemical Composition of Zhangbang Nine Steaming Nine Sun-dying Rehmanniae Radix Based on UPLC-Q-TOF-MS Technique and Study on the Function of Solid Excipiemts in Alleviating the "Nourishing and Spleen-impairing" in Rehmanniae Radix

摘要

摘要:
目的利用UPLC-Q-TOF-MS技术研究樟帮九蒸九晒地黄的化学成分及固体辅料缓解地黄“滋腻碍脾”的功能。
方法采用UPLC-Q-TOF-MS对樟帮九蒸九晒地黄进行数据采集，利用UNIFI平台及自建数据库对樟帮九蒸九晒地黄化学成分进行快速定性分析及鉴定；通过建立小鼠胃动力低下模型，测定其胃残留率和小肠推进率，研究固体辅料对地黄“滋腻碍脾”功能的影响。
结果通过UPLC-Q-TOF-MS和UNFI平台鉴定出九蒸九晒地黄中化学成分76个，包括9个环烯醚萜苷、11个苯乙醇苷、31个黄酮、6个糖类化合物、19个其他化合物；对小鼠胃排空和小肠推进作用实验结果显示，樟帮九蒸九晒地黄具有胃排空和肠推进作用。
结论该方法可用于九蒸九晒地黄的快速鉴别分析，九蒸九晒地黄中固体辅料陈皮、砂仁可以缓解地黄的“滋腻碍脾”功能。本研究可为樟帮特色饮片九蒸九晒地黄的质量控制和药效物质基础的阐明提供参考。

Abstract:
OBJECTIVE To study the chemical composition and the function of solid excipients in relieving the "nourishing and spleen-impairing" effect of Zhangbang nine steaming nine sun-dying Rehmanniae Radix(RR) by using UPLC-Q-TOF-MS technique.
METHODS UPLC-Q-TOF-MS was used to collect the data of Zhangbang nine steaming nine sun-dying RR, and UNIFI platform and self-built database were used for the rapid qualitative analysis and identification of the chemical components of Zhangbang nine steaming nine sun-dying RR. The effect of solid excipients on the function of RR "nourishing and spleen-impairing" was investigated by establishing a mouse model of low gastric motility and measuring its gastric residual rate and small intestine propulsion rate.
RESULTS The UPLC-Q-TOF-MS and UNFI platforms identified 76 chemical components in nine steaming nine sun-dying RR, including 9 iridoid glycosides, 11 phenylethyl glycosides, 31 flavonoids, 6 saccharides and 19 other compounds; the results of gastric emptying and small intestinal propulsion experiments on mice showed that Zhangbang nine steaming nine sun-dying RR had gastric emptying and intestinal propulsion effects.
CONCLUSION This method can be used for the rapid identification and analysis of nine steaming nine sun-dying RR, which has solid excipients Citri Reticulatae Pericarpium and Amomi Fructus to alleviate the "nourishing and spleen-impairing" function of RR. This study can provide a reference for quality control and elucidation of the material basis of the medicinal effects of specialty beverage of Zhangbang nine steaming nine sun-dying RR.

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