Abstract:
OBJECTIVE To profile and characterize the ingredients absorbed into blood and their metabolites of Qige decoction in hyperlipidemia rats, and so as to provide a reference for investigation of the pharmacodynamicsubstances of Qige decoction.
METHODS Rats model with hyperlipidemia induced by high-fat diet feeding were intragastrically administered with Qige decoction, and the rat plasma samples were collected and analyzed by UHPLC-Q-Orbitrap HRMS to identify the prototype ingredients absorbed into blood and their metabolites.
RESULTS A total of 46 chemical components were identified, of which 15 were prototype components and 31 were metabolites. Among the 15 prototype components, 7 were from Astragali Radix, 5 were from Puerariae Lobatae Radix, 3 were from Citri Reticulatae Chachiensis Pericarpium, the 31 metabolites were mainly derived from complex metabolites, which contained structural units including calycosin, calycosin 7-O-glucoside, genistein, puerarin, daidzein, nobiletin and hesperetin. The main metabolic pathways were hydroxylation, reduction reaction, oxidation reaction, hydrolysis reaction, demethylation reaction, uronidation and its complex reaction, glucuronidation and its complex reaction.
CONCLUSION The main metabolic pathways of calycosin, calycosin 7-O-glucoside, genistein, puerarin, daidzein, nobiletin and hesperetin in rats are phase Ⅰ and phase Ⅱ metabolism. This method can quickly analyze the ingredients absorbed into blood and their metabolites of Qige Decoction.
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