Abstract: OBJECTIVE To establish an analytical method for the simultaneous determination of 34 plant growth regulators residues in Angelicae Dahuricae Radix based on UHPLC-MS/MS. METHODS The samples were extracted by acetonitrile high-speed homogenization, concentrated, injected directly without purification method, separated by a Waters Acquity UPLC HSS T3(2.1 mm×150 mm, 1.8 µm) column with mobile phase of 0.1% formic acid aqueous solution containing 10 mmol·L^-1 ammonium formate(A)-acetonitrile(B). The matrix-matched external standard method was used for quantitative analysis. RESULTS The calibration curves of 34 plant growth regulators showed good linearity with correlation coefficients >0.995. The limits of detection were 0.01-9.26 µg·kg^-1, and average recoveries of 72.4%-100.6%, the RSD <15%. The plant growth regulators in 35 batches of Angelicae Dahuricae Radix were analyzed by this method. CONCLUSION The established method is sensitive, accurate, targeted, and simple with rapid pre-treatment, and can be used for screening and detection of plant growth regulator multi-residues in Angelicae Dahuricae Radix.