Abstract: OBJECTIVE To establish a UHPLC-MS/MS method for simultaneous determination of five ginsenosides in Shengui lurong pills. METHODS The determination was performed on Agilent C₁₈(100 mm×2.1 mm, 1.8 μm) column with column temperature of 25 ℃. Methanol and 0.1% aqueous formic acid solution were used as mobile phase for gradient elution with the flow rate of 0.3 mL·min^-1. Ion source was electrospray ion source, positive ion mode monitoring was conducted and MRM mode was adopted. RESULTS The linear ranges of ginsenoside Re, Rg₁, Rf, Rb₁ and pseudo-ginsenoside F₁₁ had good resolution and linear relationships(r²≥0.999 6). The spiked recoveries were in the range of 96.3%-104.9% with RSD(n=9)<3.0%. RSDs of instrument precision, stability and reproducibility tests were <3.0%. CONCLUSION The method is simple, accurate, reliable, and can be uesd for quality control and evaluation of Shengui Lurong pills.