Abstract: OBJECTIVE To evaluate the mouse model of acute pneumonia induced by lipopolysaccharide inhalation constructed by simple atomization equipment. METHODS After lipopolysaccharide atomization inhalation, the changes of body weight, total protein, nitric oxide(NO) and leukocyte levels in lung lavage fluid of mice were observed at different time points. At the same time, HE, Masson and TUNEL staining were performed on the lung tissue for pathological detection. Observed the recovery of animals after model construction. RESULTS Compared with the control group, the body weight of the model mice decreased significantly(P<0.01), the total protein level and the number of white blood cells in the lung lavage fluid significantly increased(P<0.01), and the NO level significantly decreased(P<0.01) after modeling. The results of lung pathological examination showed that the lung tissue of the model group was seriously damaged, fibrosis occurred, and a large number of cells apoptosis, which was significantly different from that of the control group. CONCLUSION The mouse model of acute pneumonia induced by lipopolysaccharides can be successfully constructed by using a simple atomization. The model is relatively stable and can be used to screen the drug efficacy.