Abstract: OBJECTIVE To investigate the protective effects of water-extracted Polygonum multiflorum polysaccharides (WPMP) on systemic inflammation induced by lipopolysaccharide(LPS) in zebrafish. METHODS Two components of WPMP were isolated and purified(WPMP-1 and WPMP-2). Zebrafish embryos were collected and cultured at 28.5℃. Blank group and LPS-induced model group were set up. The optimal induction concentration of LPS was determined by hatchability, heart rate, yolk sac size, reactive oxygen species(ROS) production rate and NO production rate, and after the establishment of inflammatory model, the anti-inflammatory effects of WPMP were evaluated by the above indexes. RESULTS Different concentration of WPMP(10, 50, 100 μg·mL^-1) had a concentration-dependent protective effect on inflammation induced by LPS, and could significantly reduce the size of yolk sac, heart rate, ROS production rate and NO production rate. Under the same polysaccharide concentration, the anti-inflammatory activity of WPMP-2 was higher than that of WPMP-1. CONCLUSION WPMP has good anti-inflammatory effect, which provides scientific basis for WPMP as a natural anti-inflammatory component.