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    中华跌打丸调节钙通道及ATP代谢改善外周疲劳作用研究

    Study on Anti-peripheral Fatigue Effect of Zhonghua Dieda Pills by Regulating Calcium Channel and ATP Metabolism

      摘要
    • 摘要: 目的 建立缺氧-疲劳斑马鱼模型,研究中华跌打丸抗外周疲劳作用及其机制。方法 利用亚硫酸钠诱导建立缺氧-疲劳模型,分别从运动能力、乳酸含量、ATP生成、镇痛、自由基清除和钙离子代谢6个方面考察中华跌打丸的抗疲劳作用。结果 中华跌打丸(250,500,1 000和2 000 µg·mL-1)可明显改善疲劳斑马鱼的运动能力,促进乳酸代谢,降低机体乳酸含量,同时具有镇痛作用;中华跌打丸1 000 µg·mL-1可明显促进疲劳斑马鱼ATP能量合成;中华跌打丸500,1 000和2 000 µg·mL-1可明显提高超氧化物歧化酶1(SOD1)基因相对表达量;中华跌打丸1 000,2 000 µg·mL-1可明显提高钙离子通道(CACNAIDA)基因相对表达量。结论 中华跌打丸具有明显的抗外周疲劳和镇痛作用,其机制可能与促进乳酸代谢、促进ATP生成、提高SOD1和CACNA1DA基因相对表达量有关。

       

      Abstract: OBJECTIVE To establish the zebrafish hypoxia-fatigue model, and to study the anti-peripheral fatigue effect of Zhonghua Dieda pills and its mechanism. METHODS The hypoxia-fatigue model of zebrafish was established by sodium sulfite. The anti-fatigue effects of Zhonghua Dieda pills were observed from six aspects:exercise, content of lactic acid, ATP production, degree of pain, free radical scavenging and calcium ion metabolism. RESULTS Zhonghua Dieda pills(250, 500, 1 000 and 2 000 µg·mL-1) could significantly improve the exercise ability of fatigued zebrafish, promote lactic acid metabolism, and reduce the content of lactic acid. Zhonghua Dieda pills 1 000 µg·mL-1 could significantly promote ATP energy synthesis of fatigued zebrafish, while having an analgesic effect. Zhonghua Dieda pills 500, 1 000 and 2 000 µg·mL-1 could significantly increase the relative expression of SOD1 gene. Zhonghua Dieda pills 1 000, 2 000 µg·mL-1 could significantly increase the relative expression of CACNA1DA gene. CONCLUSION Zhonghua Dieda pills has obvious anti-peripheral fatigue and analgesic effect, the mechanism maybe related to promoting lactate metabolism, promoting ATP production, increasing the relative expression of SOD1 gene and increasing the relative expression of CACNA1DA gene.

       

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