Abstract: OBJECTIVE To prepare the standard decoction of Codonopsis Radix and conduct quality standard research. METHODS Twenty-eight batches of Codonopsis Radix were collected and the standard decoctions were prepared by using water to extract for 2 times. The investigations were on the extraction rate, content of lobetyolin and polysaccharides. The HPLC fingerprint was determined. RESULTS The extraction rate of 28 batches of Codonopsis Radix standard decoction was 23.94%-47.63%. The content of lobetyolin was 0.059 4-0.329 8 mg·g^–1. The content of polysaccharides was 0.059 4-0.173 4 g·g^–1. The fingerprint of 28 batches of Codonpsis Radix were compared and analyzed with thirteen common peaks in which lobetyolin was identified by the reference. CONCLUSION In this study, the standard decoctions of Codonopsis Radix are prepared with standardized method and the quality is stable. The analysis methods are good for the instrument precision, stability and repeatability, which can provide reference for the establishment of the quality standard of Codonopsis Radix formula.