Abstract: OBJECTIVE To detect Cervi Cornu specific peptides components,provide an experimental basis for the quality standard investigation on Cervi Cornu,Cervi Cornu Colla and their products. METHODS By using ultrasonic assisted trypsin digestion method,Cervi Cornu,and Cervi Cornu Colla samples were prepared rapidly.High performance liquid chromatography with triple-quadrupole mass spectrometry was used to detect the Cervi Cornu and Cervi Cornu Colla samples.Under positive electrospray ionization mode and multiple reaction monitoring,ion pair of m/z 765.5→554.4,m/z 850.4→515.4,m/z 864.0→535.4,m/z 858.7→527.4,m/z 612.2→464.2 and m/z 845.0→507.3 were used to detect deer specific peptides in Cervi Cornu,Cervi Cornu Colla or adulterated Cervi Cornu Colla samples.RESULTS The determination of deer specific peptides in Cervi Cornu samples could be achieved in 1 h used the present method,which could be used to distinguish Cervi Cornu and deer skin samples,and the detection of adulterated Cervi Cornu Colla samples.CONCLUSION Ultrasonic assisted digestion combined with tandem mass spectrometry is fast,accurate and specific.It can be used for the rapid and accurate detection of Cervi Cornu,Cervi Cornu Colla and their products.