Abstract: OBJECTIVE To explore the effect of Shenxiao Tuolisan on CCl₄-induced hepatic fibrosis in rats and its possible mechanism. METHODS The rat hepatic fibrosis model was established by intragastric administration of 40% CCl₄(2 mL·kg^–1),twice a week for 8 weeks.After the intervention of Shenxiao Tuolisan at 6 week for 8 weeks.Evaluated anti-fibrotic efficacy by organ index and Masson staining.HPLC-QTOF-MS combined technology was used to detect the changes of metabolites in the plasma of rats in each group.Ropls software was used to partial least squares discriminant analysis,differential metabolite screening and metabolic pathway analysis.RESULTS The organ index results showed that Shenxiao Tuolisan could reduce the liver index and spleen index,increase the thymus index in different degrees,and there was a significant difference compared with the model group (P<0.01).The results of Masson staining showed that the hyperplasia of fibrous connective tissue in the liver became shallower and narrower after the intervention of Shenxiao Tuolisan.Metabolomics analysis indicated that,compared to the model control group,385 metabolites changed after the intervention of Shenxiao Tuolisan,of which 20 were up-regulated and 365 were down-regulated,such as arachidonate acid,cholic acid,linoleate acid.Mainly related to regulation of lipolysis in adipocytes,linoleic acid metabolism,bile secretion,sphingolipid signaling pathway,biosynthesis of unsaturated fatty acids.CONCLUSION Shenxiao Tuolisan has a good effect on CCl₄-induced hepatic fibrosis in rats,and its mechanism of action may be related to the regulation of lipolysis in adipocytes,bile acid synthesis and other metabolic pathways.