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    一测多评法同时测定蒙药吉如合-6中6种成分含量

    Simultaneous Determination of Six Components in Mongolian Medicine Jiruhe-6 by Quantitative Analysis of Multi-components by Single-marker

      摘要
    • 摘要: 目的 利用一测多评法同时测定蒙药吉如合-6中鞣花酸、肉桂酸、丁香酚、木香烃内酯、去氢木香内酯、去氢二异丁香酚等6种成分的含量，并验证该方法在蒙药吉如合-6质量控制研究中的可行性与科学性。方法 采用一测多评法；供试品采用75%甲醇回流提取；以丁香酚为内标物，测定其他5种成分相对校正因子(ƒk/m)；采用一测多评法与外标法进行含量计算、比对。结果 所测定的鞣花酸、肉桂酸、丁香酚、木香烃内酯、去氢木香内酯、去氢二异丁香酚等6种成分分别在其各自范围内有良好的线性关系(即r>0.999 9)，平均加样回收率为94.80%~98.85%，RSD<2.0%，外标法与一测多评法所测得结果之间相对误差<2%。结论 一测多评法操作简便、准确可行、重复性好，可用于蒙药吉如合-6的质量控制。

       

      Abstract: OBJECTIVE To determine the ellagic acid,cinnamic acid,eugenol,costunolide,dehydrocostus lactone and dehydrodiisoeugenol in Mongolian medicine Jiruhe-6 by quantitative analysis multi-components by single marker (QAMS).And to verify the feasibility and scientificity of this method in the quality control study of Mongolian medicine Jiruhe-6.METHODS QAMS method was used.The test sample was extracted with 75% methanol under reflux.Relative calibration factors (ƒk/m) of other 5 components were determined with eugenol as internal standard substance.QAMS and external standard method were used for content calculation and comparison.RESULTS There was a good linear relationship between ellagic acid,cinnamic acid,eugenol,costunolide,dehydrocostus lactone and dehydrodiisoeugenol in their respective ranges (r>0.999 9).The average recovery was 94.80%-98.85%,RSD<2.0%.The relative error between external standard method and QAMS was<2%.CONCLUSION QAMS method is simple,accurate,feasible and reproducible.It can be used for the quality standard control of Mongolian medicine Jiruhe-6.

       

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