Abstract: OBJECTIVE To explore the Chinese medicine clinical prescription rules and molecular mechanism of Angelicae Sinensis Radix for chronic cough under the guidance of the traditional Chinese medicine theory of "Treating Cough and Reversing Upper Qi". METHODS Relevant databases were searched to collect Angelicae Sinensis Radix prescription for the clinical treatment of chronic cough. Statistical analysis was carried out on the frequency, nature and flavour and meridian tropism, four properties and five flavors and their efficacy of Angelicae Sinensis Radix prescriptions. And the traditional Chinese medicine of high frequency was analyzed systematically by cluster analysis. Use TCMSP and other databases to screen the components and targets contained in Angelicae Sinensis Radix, the targets of chronic cough disease and the intersection targets of Angelicae Sinensis Radix in the treatment of chronic cough. The intersection targets were used to analyze the protein-protein interaction network and KEGG pathway and construct components-targets-pathway networks. Using molecular docking to verify the blinding activity between the key components and targets obtained by topological analysis, animal experiments were done to verify cough suppression of ferulic acid based on phosphatidylinositol 3-kinase receptor 1(PIK3R1). RESULTS Four hundred and twenty-three Angelicae Sinensis Radix prescriptions for the treatment of chronic cough were collected, covering 419 traditional Chinese medicines, of which the most common were tonic, phlegm-relieving, cough-relieving and asthma-relieving drugs, most of which were related to the lung, spleen, and liver meridians, and mainly warm, cold, flat, sweet, bitter and acrid; the search obtained 41 active ingredients of Angelicae Sinensis Radix, 157 targets and 164 pathways of Angelicae Sinensis Radix in the treatment of chronic cough. The key components of Angelicae Sinensis Radix had good binding activity in the molecular docking of key targets, and the key component of Angelicae Sinensis Radix ferulic acid could significantly prolong the cough latency of mice, reduce the number of coughs and reduce the expression levels of PIK3R1 and its genes in lung tissue. CONCLUSION In clinical practice, Angelicae Sinensis Radix is often combined into compound prescriptions for the treatment of chronic cough. It exerts a therapeutic effect by acting on multiple components and multiple targets. The ferulic acid contained in Angelicae Sinensis Radix exerts an antitussive effect by inhibiting PIK3R1.