Abstract: OBJECTIVE To study the regulatory effect of Chen's Peiyuan decoction medicated serum on autophagy of ovarian granulosa cells(OGCs). METHODS The SD rats were divided into normal group and Chen's Peiyuan decoction group, then collected the drug medicated serum. The isolated OGCs were divided into normal group, model group, Chen's Peiyuan decoction group and signal pathway blocker group. Without the normal group, the other three groups were made immune premature ovarian failure models through tripterygium glycosides. The normal and model groups were added with normal group rat serum, Chen's Peiyuan decoction group was added with Chen's Peiyuan decoction medicated serum, and the signal pathway blocker group was added Chen's Peiyuan decoction medicated serum and signal pathway blocker. The E₂ and P in group culture medium were determined by ELISA. The expression of LC3II, m-TOR, p62 in cells were analyzed by immunofulourescence staining. The autophagy of OGCs were observed by transmission electron microscope. The relative expression of Beclin-1, LC3-II, m-TOR and p62 were measured by Western blotting. RESULTS Compared with the normal group, the E₂ concentration in the model group and signal pathway blocker group were obviously decreased(P<0.05). The E₂ concentration in Chen's Peiyuan decoction group was higher than model group and signal pathway blocker group(P<0.05). There was no significant difference in the concentration of P in the normal group, model group, Chen's Peiyuan decoction group and signal pathway blocker group. In normal group and Chen's Peiyuan decoction group, the LC3II had weak immunofluorescence expression, but m-TOR and p62 had enhanced immunofluorescence expression. In model group and signal pathway blocker, the LC3II had enhanced immunofluorescence expression, but m-TOR and p62 had weak immunofluorescence expression. A large number of autophagy bodies were observed in granulosa cells in model and signal pathway blocker groups while the normal and Chen's Peiyuan decoction groups were seldom. Compared with model group and pathway blocker group, the relative expression levels of LC3-II and beclin-1 in Chen's Peiyuan decoction group were decreased, while the relative expression level of m-TOR and p62 protein increased(P<0.05). CONCLUSION The Chen's Peiyuan decoction can effectively inhibit the transitional autophagy of OGCs and protect ovarian.