Abstract: OBJECTIVE To explore the spectrum effect relationship of the volatile oil of Qizhi Weitong granules in promoting intestinal motility, and to provide experimental basis for the quality control of Qizhi Weitong granules. METHODS The GC-MS fingerprint of the volatile oil of Qizhi Weitong granules was established. The pharmacological effect of the volatile oil on promoting intestinal motility was determined by the method of semi-solid paste charcoal powder propulsion, and the spectral effect model of characteristic fingerprint peak and promoting intestinal motility was established by grey correlation analysis combined with partial least square regression. RESULTS There were 44 common peaks in the fingerprints of 8 different compatibility groups, among which octanal, D-limonene, cis-carveol, carvone, Cypera-2,4-diene, β-vatirenene, cyperene, β-copaene, spathulenol, 6-isopropenyl-4,8a-dimethyl-1,2,3,5,6,7,8,8a-octahydro-naphthalen-2-ol were potential pharmacodynamic substances. CONCLUSION This study provides experimental basis for determining the quality control index components of Qizhi Weitong granules in promoting intestinal motility.