Abstract: OBJECTIVE To establish working mechanism for mill-based quality supervision of vaccine manufacturers. METHODS An effective inspection mechanism was established by combining the analysis of the vaccine inspection system and the working practice. RESULTS A working mechanism was established from two aspects:the working method and the communication mechanism of the mill-based quality supervision, and the problems encountered in the work were also discussed. CONCLUSION The established inspection mechanism has been effectively used in the actual inspection work, providing reference for the follow-up inspection work of vaccine manufacturers.