Abstract: OBJECTIVE To analyze and predict the Q-marker of Shujin Huoxue tablets(SHT) based on fingerprint and network pharmacology. METHODS The fingerprints of SHT were established and analyzed by using the Similarity Evaluation System Software for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 edition). The network pharmacology was used to screen and analyze the function targets and pathways of related components of SHT. The "ingredients-targets-pathways" network was constructed to predict the potential Q-marker of SHT. RESULTS The fingerprints of SHT were established through representative samples, and the similarity was determined. The similarity of some batches were <0.8, which may be caused by the difference of production process. Nine common ingredients were identified including 5-hydroxymethylfurfural, protocatechuic acid, protocatechuic aldehyde, syringin, chlorogenic acid, hydroxysafflor yellow A, tracheloside, 4-methoxysalicylaldehyde and periplocin. A total of 9 active ingredients, 30 core target sites and 10 key pathways of SHT were screened out through network pharmacology system. Hydroxysafflor yellow A, protocatechuic acid, protocatechuic aldehyde, syringin, tracheloside and 4-methoxysalicylaldehyde were predicted as potential Q-marker based "Five Principles" of Q-marker. CONCLUSION In this study, the Q-marker of SHT are analyzed by fingerprint and network pharmacology, which provides reference for quality control of SHT, and for further study on the mechanism of SHT.