Abstract: OBJECTIVE To investigate the influence of the powder mesh and the percutaneous absorption enhancer on the percutaneous penetration of the traditional Chinese medicine Gushang gel patch. METHODS Using hydroxysafflor yellow A and dracorhodin as evaluation indicators, the paddle method was used to evaluate the in vitro release behavior of the gel patch, and the Franz diffusion cell method was used to investigate the effect of powder mesh and transdermal absorption enhancers on the transdermal absorption of gel patch. RESULTS Compared with azone and menthol, isopropyl myristate had the best penetration promoting effect on hydroxysafflor yellow A and dracorhodin. The optimal dosage was 3%, 200 mesh powder had no significant effect on the in vitro release and transdermal absorption of active components compared with 80 mesh powder. CONCLUSION The powder mesh has no significant effect on the transdermal penetration of the gel patch whereas the transdermal absorption enhancer of isopropylmyristate can improve the transdermal absorption of the gel patch obviously.