高级检索

    GC-MS同时测定复方鲜竹沥液中8个酚类有效成分

    Simultaneous Determination of 8 Phenolic Active Components in Fufang Xianzhuli Ye by GC-MS

      摘要
    • 摘要: 目的 建立GC-MS同时测定复方鲜竹沥液中苯酚、愈创木酚、甲基愈创木酚、乙基愈创木酚、乙烯基愈创木酚、2,6-二甲氧基苯酚、香兰素和丁香醛8个酚类有效成分含量的方法。方法 样品经乙醚萃取后,采用DB-35MS UI色谱柱(30 m×0.25 mm,0.25 μm)进行分离,采用离子监测模式(SIM)进行质谱检测,外标法定量。结果 在优化实验条件下,8种酚类成分分离良好,在0.1~129.4 μg·mL-1内线性良好,相关系数均>0.999 0,方法检出限均<0.043 μg·mL-1;在3个不同加标浓度下的回收率为93.76%~101.33%,RSD值均≤ 3.08%。结论 该方法简单、灵敏、准确并且可靠,可为复方鲜竹沥液的质量控制提供科学依据。

       

      Abstract: OBJECTIVE To establish a GC-MS method for the simultaneous determination of 8 phenolic active components such as phenol, guaiacol, 2-methoxy-4-methylphenol, 4-ethyl-2-methoxy-phenol, 4-hydroxy-3-methoxy-styrene, 2,6-dimethoxyphenol, vanillin and syringaldehyde in Fufang Xianzhuli Ye. METHODS The samples were extracted with ether and separated by a DB-35MS UI column(30 m×0.25 mm, 0.25 μm), then the extract was detected by MS under SIM mode. And external standard method was used for quantification. RESULTS Under the optimized experimental conditions, 8 phenolic components were well separated. The linearity was good within the mass concentration range of 0.1-129.4 μg·mL-1, correlation coefficients were >0.999 0. The detection limits of phenolic active components were <0.043 μg·mL-1. The spiked recoveries were in the range of 93.76%-101.33% with RSD ≤ 3.08%. CONCLUSION The method is simple, sensitive, accurate and reliable, and can provide some scientific basis for quality control of Fufang Xianzhuli Ye．

       

      • HTML全文
      • 参考文献(16)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回