Abstract: OBJECTIVE To establish a UPLC method for the simultaneous determination of 7 active ingredients in different parts of Tetrastigma hemsleyanum of different origins. METHODS UPLC was used for separation and detection. The chromatographic analysis was performed on BEH Shield RP18 column(150 mm×2.1 mm, 1.7 μm). The column temperature was 35℃. The injection volume was 2 μL. The mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) at the flow rate of 0.3 mL·min^-1 with gradient elution. The detection wavelengths were 360 nm and 280 nm. The contents of 7 kinds of active ingredients were determined by external standard method. RESULTS Catechin, procyanidin B1, rutin, isoquercetin, kaempferol-3-o-rutoside, astragaloside and apigenin showed a good linear relationship in 1.02-40.8, 1.43-57.0, 1.16-46.2, 1.09-43.6, 1.12-44.6, 1.18-47.2, 0.30-11.8 μg·mL^-1, respectively. There were some differences in the contents of various componennts in different areas and different parts of T. Hesleyanur. CONCLUSION The established UPLC method for the simultaneous determination of 7 active ingredients in Tetrastigma hemsleyanum is accurate and feasible, which provides a reference for the quality control of tetrastigmae radix.