Abstract: Exosomes are extracellular vesicles with a diameter of 30-100 nm secreted by various cell types. Exosomes are composed of lipid bilayer membranes and membrane-associated proteins, contain cytoplasmic components such as mRNA, miRNA, and proteins. Exosomes can bind to or fuse with the membrane of the target cell to transport surface proteins and cytoplasmic components into the cell. Exosomes participate in cell-to-cell communication by transferring their bioactive substances to distant cells and are thought to regulate gene and protein expression levels in recipient cells. Exosomes are involved in many stress conditions such as hypoxia, senescence and oncogene activation. Exosomes can be isolated from cell media or body fluids by differential centrifugation, filtration plus centrifugation, immunoaffinity or exclusion chromatography, polymer precipitation and microfluidic techniques. However, the preservation conditions of exosomes from different sources have not been determined, the stability of the preservation conditions and the subsequent experimental study of exosomes are very important. Based on this, this review summarized the research progress of suitable conditions for exosome preservation as follows to provide reference for the subsequent research.