Abstract: OBJECTIVE To establish the characteristic chromatogram of monosaccharides in Ginkgo Armillaria oral solution by using HPLC and to evaluate the quality control methods of Ginkgo Armillaria oral solution from different sources, METHODS The monosaccharides in 4 batches of commercial Ginkgo Armillaria oral solution and 6 batches of self-made oral solution were analyzed by 1-phenyl-3-methyl-5-pyrazolone(PMP) precolumn derivatization HPLC combined with traditional Chinese medicine fingerprint similarity analysis software. At the same time, the common peaks were assigned and confirmed by measuring the composition of monosaccharides in the formula. RESULTS By studying the HPLC characteristic chromatography of the self-made oral solution and the commercially available oral solution, five common peaks were identified. After attribution verification, they were mannose, rhamnose, glucose, galactose, and arabinose. The similarity was between 0.998 and 1.000; at the same time, the monosaccharide composition of the oral solution and its ingredients was determined, and it was found that the monosaccharide in the hydrolyzed oral solution was derived from Armillaria mellea powder, Ginkgo biloba extract and the flavoring agent stevioside; The monosaccharide composition of Ginkgo biloba extract and Armillaria mellea powder from different manufacturers was determined, and the results showed that the monosaccharide composition of Ginkgo biloba extract and Armillaria mellea powder from different manufacturers was significantly different. Finally, through the extraction and determination of the polysaccharides in the oral liquid and its raw and auxiliary materials, it was found that the polysaccharides in the oral liquid were mainly derived from Armillaria mellea powder. CONCLUSION The method established in this study can comprehensively reflect the characteristics of monosaccharides in components of Ginkgo Armillaria oral solution. The hydrolyzed monosaccharides of the commercially available oral solution, self-made oral solution and its original medicinal materials can be attributed and traced, which can be used for the quality evaluation of oral solution, raw medicinal materials and recipes.