Abstract: OBJECTIVE To explore the biological function and related mechanism of zinc finger protein 403(ZFP403) in ovarian epithelial cell.METHODS ZFP403 shRNA lentivirus was constructed and transfected into human ovarian epithelial cell line IOSE80 and screened by puromycin; qRT-PCR and Western blotting were used to detect the silence effect of ZFP403, respectively; the cell growth ability after knockdown of ZFP403 was evaluated by plate colony formation assay; soft agar colony formation assay was used to evaluate the anchorage independent growth ability; the effect of ZFP403 on cell cycle was detected by flow cytometry; Western blotting was used to study the mechanism of ZFP403 on cell cycle related proteins.RESULTS ZFP403 stably silenced cell lines(IOSE80-ZFP403-shRNA1, 2) and negative control cell line(IOSE80-shRNA-NC) were successfully constructed after puromycin screening; flow cytometry showed that knockdown of ZFP403 could significantly promote the growth and anchorage independent growth in IOSE80 cells; Western blotting showed that knockdown of ZFP403 could significantly promote cell cycle progression; the expression of p-cdc2, p21 was downregulated and cdc2, cyclinB1 upregulated after ZFP403 knockdown.CONCLUSION ZFP403 promotes the abnormal growth of ovarian epithelial cells. The expression level of ZFP403 may affect the transformation of ovarian epithelial cells to ovarian cancer, which may be used as a clinical diagnostic marker for early ovarian cancer.