Abstract: OBJECTIVE To simultaneous determine 22 illegally drugs added in uric acid regulating traditional chinese medicine and chinese herbal food by HPLC-MS/MS. METHODS The samples were extracted by methanol mixed with 0.1% ammonium hydroxide and separated on an Agilent Poroshell 120 Bonus-RP column(2.1 mm×100 mm, 2.7 μm), with methanol-acetonitrile(1:1) and 0.005 mol·L^-1 ammonium formate as mobile phases by gradient elution. The analytes were detected by electrospray ionization source in the positive or negative mode and the multiple reaction monitoring mode. RESULTS the calibration curves for 22 drugs were linear in the range of 10.0-200 μg·L^-1 with their correlation coefficients r²>0.99, the limits of detection and quantitation were in the ranges of 0.125-0.750 mg·kg^-1 and 0.416-2.81 mg·kg^-1 respectively. The average recoveries were in the ranges of 80.2%-120.1%, with RSDs of 1.1%-5.9%. Illegal added drugs were not found in 37 tested practical samples. CONCLUSION The established method is simple, rapid and sensitive, and can be used for the simultaneous of determination of 22 illegally drugs added in uric acid regulating traditional Chinese medicine and Chinese herbal food.