Abstract: OBJECTIVE To establish a GC-MS method for simultaneous determination of 34 kinds of antimicrobial agents and antioxidants in Chinese herbal extracts. METHODS Using Agilent DB-5MS capillary column(60 m×320 μm, 0.5 μm); injection port temperature:250℃; carrier gas:high purity helium; flow rate:2 mL·min^-1; injection volume:1 μL; column temperature:40℃, maintained for 1 min, rose to 300℃ at a rate of 5℃·min^-1, maintained for 10 min; ionization source was EI source, the multi-reaction monitoring mode was used for qualitative and quantitative analysis. RESULTS The results showed that the linear range of 34 kinds of antimicrobial agents and antioxidants in a certain concentration range was great, and the correlation coefficients were all > 0.99. The recoveries of each component in the extractum ranged from 83.7% to 124.8%, and RSD(n=6) were between 0.5% and 5.2%. The recoveries in extract powder ranged from 71.3% to 119.6%, and the RSD(n=6) ranged from 0.7% to 5.6%. CONCLUSION The sensitivity, precision and recovery of the method can meet the instrument requirements of the detection of antibacterial agents and antioxidants in Chinese herbal extracts.