Abstract: In recent years, with the rise of the 3R principle of experimental animals, the research of animal replacement experiments has become more and more significant. In order to keep the development of domestic cosmetics in line with international standards, China has accelerated the application of alternative experimental methods in the safety testing of cosmetics. In view of the possible irritation, sensitization, phototoxicity and other adverse reactions of cosmetics to the skin, this paper summarizes the alternative methods and integrated approaches to testing and assessment(IATA) for the toxicological end point of cosmetic skin toxicology. At the same time, this paper also provides an important basis for the application of three-dimensional reconstituted skin model and model biological zebrafish in dermal toxicity test of cosmetics.