Abstract: OBJECTIVE To establish the U-SENS^™ test method which is in line with the actual situation of China, and verify the reliability of the new test method. METHODS Refer to U-SENS™ test method in OECD guideline 442E Appendix II, a test system with different concentrations of abietic acid(20, 30, 40, 50, 60, 100 μg·mL^-1) as the positive agent was established for 10 tests according to the QC template, the optimum positive concentration of human lymphoma cell line U937 was explored by flow cytometry. And eight compounds with known sensitization, such as saccharin, p-phenylenediamine and cinnamyl alcohol were verified. RESULTS The 40, 50 μg·mL^-1 abietic acid could show stable positive reaction under the condition with low cytotoxicity, and could correctly classify 8 compounds with known sensitization. CONCLUSION U-SENS^™ test system is established with abietic acid as positive agent, and 40 or 50 μg·mL^-1 can be chosen as the positive control concentration to ensure that the test system can accurately classify the skin sensitization of the test object.