Abstract: The discovery of a new drug with clinical efficacy usually requires the screening of thousands of compounds one by one. When the target compound is obtained, it needs to be further verified. This process requires a lot of work and a long cycle. The emergence of high throughput and high content screening technology has shortened the time to find effective drugs, and the selection of appropriate biological models has improved the accuracy of screening results. This article mainly reviews the applications of stem cell biological models, CRISPR/Cas9 gene editing cell models, and 3D cell culture models in high throughput and high content drug screening, and briefly describes the application of these methods in the research and development of drugs for the treatment of women's malignant tumors.