Abstract: Spiders are derived from wild animal resources, and their toxins and spider silk have strong biological activities, which are widely used in the field of biology and material engineering. In this paper, research literature on the structure and pharmacology effect of spider venom and spider silk at home and abroad are summarized and analyzed, focus on the research progress of spider toxin on ion channel, neuroprotection, anti-tumor, antibacterial and enzyme activity in recent years. And the application of spider silk in procoagulant, nerve repair and composite carrier are reviewed as well. It will provide a reference for the further research on spider toxin and spider silk, and lay a foundation for the development of artificial breeding and new drug research.