Abstract: Whole embryo culture(WEC) technique in vitro is one of the most important methods to study the pharmacological toxicology and mechanism of drugs and chemicals during embryonic development, which is used to cultivate animal embryos in a controlled in vitro environment. In this paper, WEC technique and its recent research progress were reviewed from the perspectives of the development of rats and rabbits WEC, the culture conditions, the scoring system for embryonic development endpoints and the expanded embryonic development evaluation endpoints, and the applications of drug reproduction and developmental toxicology. WEC technique is one of the important alternative research methods in the research of drug reproduction and developmental toxicity, and the WEC study of the combination of rats and rabbits is more important and provides more comprehensive information for the study of embryonic developmental toxicity.