Abstract: OBJECTIVE To explore the effect of Dendrobium officinale polysaccharides on autophagy related to adenosine monophosphate-activated protein kinase(AMPK)/UNC-51 like autophagy activating kinase 1 pathway(ULK1) pathway induced by hypoxia/reoxygenation(H/R) in astrocytes. METHODS Human astrocytes were cultured in vitro and divided into control group, H/R group(H/R modeling), low concentration Dendrobium officinale polysaccharide group(H/R modeling+100 μg·mL^-1 Dendrobium officinale polysaccharide), medium concentration Dendrobium officinale polysaccharide group(H/R modeling+ 200 μg·mL^-1 Dendrobium officinale polysaccharide) and high concentration Dendrobium officinale polysaccharide group(H/R modeling+400 μg·mL^-1 Dendrobium officinale polysaccharide). MTT assay was used to detect cell proliferation. Flow cytometry was used to detect apoptosis. The kit was used to determine the levels of malondialdehyde(MDA) and superoxide dismutase(SOD) in cells. Western blotting was used to detect the expression of p-AMPK, AMPK, p-ULK1, ULK1, Beclin1, microtubule-associated protein 1 light chain 3(LC3) I and LC3 Ⅱ proteins. RESULTS Compared with control group, the survival rate of astrocytes, the level of SOD and the protein expression level of LC3 I/LC3 Ⅱ were significantly lower in H/R group(P<0.05), the apoptosis rate, the level of MDA and the protein expression levels of p-AMPK/AMPK, p-ULK1/ULK1 and Beclin1 were significantly higher(P<0.05); with the treatment of Dendrobium officinale polysaccharide and the increase of its concentration, the survival rate of astrocytes, the level of SOD and the protein expression level of LC3 I/LC3 Ⅱ were significantly increased(P<0.05), the apoptosis rate, the level of MDA and the protein expression levels of p-AMPK/AMPK, p-ULK1/ULK1 and Beclin1 were significantly decreased(P<0.05). CONCLUSION Dendrobium officinale polysaccharides can inhibit the activation of AMPK/ULK1 pathway and autophagy of astrocytes induced by H/R, which can promote cell survival and reduce cell apoptosis.