Abstract: OBJECTIVE To improve the quality standard of Baoyuan anticancer oral liquid. METHODS TLC was used to identify Astragali Radix, Panacis Quinquefolii Radix, Hedyotidis Herba, Scutellariae Barbatae Herba and Atractylodis Macrocephalae Rhizoma and the content of Astragaloside IV was determined by HPLC-ELSD. The WondaSil C₁₈ chromatographic column(250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was consisted of acetonitrile and water with a ratio of 32:48. The column temperature was 35℃ and injection volume was 20 μL. The elution time was 60 min. Evaporative light dispersion detector was adopted with drift tube temperature being 70℃ and nitrogen flow rate being 1.5 L·min^-1. RESULTS The TLC spots of Astragali Radix, Panacis Quinquefolii Radix, Hedyotidis Herba, Scutellariae Barbatae Herba and Atractylodis Macrocephalae Rhizoma were all clear with good separation, strong specificity and no interference with the negative sample. The detection concentration of Astragaloside IV has a good linear relationship in the range of 139.01-1 112.12 μg·mL^-1 with of peak area(r=0.998 5). The RSD of precision, repeatability and stability tests all met the requirements and the average recovery rate was 101.5%(RSD=3.4%, n=9). CONCLUSION The established quality standard method is accurate, simple, specific, and sensitive, and can better control the quality of Baoyuan anticancer oral liquid.