Abstract: OBJECTIVE To improve the real-time monitoring and early warning for differential pressure, temperature and humidity in pharmacy intravenous admixture services(PIVAS), and finally realize the automatic and intelligent remote regulation of clean room environment and key equipment. METHODS By application of on-line monitoring and control system for intelligent environment based on programmable logic controller(PLC) system, the advantages and disadvantages of the system are compared with the monitoring system for traditional monitoring system. RESULTS The on-line monitoring and control system of intelligent environment was put into use in PIVAS clean room, and it could be effectively combined with PIVAS Operation Service of Intelligent Platform(PSIP), which basically realized the real-time monitoring and early warning for differential pressure, temperature and humidity, the effective tracing and analysis for historical data, the automation and intelligentization of remote differential pressure/temperature and humidity adjustment, and the status check and remote control of the ultra clean working platform of the key equipment in the clean room. The alarm records of abnormal monitoring increased from 5 times per month to 20 times per month. The treatment time of abnormal condition decreased from (48±4.5)h per time to (3±1.5)h per time.The related manpower time was shortened from (540±60)min per month to (30±10)min per month. CONCLUSION The application of on-line monitoring and control system for intelligent environment has realized the real-time monitoring and automatic control for differential pressure/temperature and humidity in each working area of PIVAS clean room. Also the status check and remote control of the ultra clean working platform. Not only effectively ensure the environmental safety and whole process traceability of PIVAS clean room, but also greatly facilitate the operation of staff.