Abstract: OBJECTIVE To establish a GC-MS/MS method for the detection of etizolam in human plasma and provide the basis for clinical drug analysis. METHODS After extraction with toluene, plasma samples were subjected to column BR-5MS capillary column(30 m×0.25 mm, 0.25 µm) with temperature programming and ion detection in multiple reaction monitoring mode. The GC-MS/MS quantitative determination of etizolam was based on retention time and ratio of ions. The quantitative analysis was based on external standard method and calibration curve. RESULTS The linear range of etizolam was 0.5-600.0 ng·mL^-1, the coefficient of correlation r was 0.995, the lower limit of quantification was 0.5 ng·mL^-1, the recoveries ranged from 70% to 110% at three spiked levels, the RSD of inter-and intra-day was <10%, the stability of method was good, which met the requirements of biological sample determination. CONCLUSION The method is sensitive, accurate, simple and rapid, and suitable for the detection of etizolam plasma concentration.