Abstract: OBJECTIVE To establish quality standards for the standard decoction of Ophiopogon japonicus pieces. METHODS Fifteen batches of Ophiopogon japonicus pieces were collected. The standard decoctions were prepared by water decoction. Methylophiopogonanone A was considered as the index component and its analysis method was established. The extraction ratio of dry matter, the yield and transfer rate of methylophiopogonanone A were calculated. The characteristic fingerprint method for the analysis of Ophiopogon japonicus standard decoctions was also established. Quality standards for the standard decoctions were developed by the x± 3SD method. RESULTS The quality standard of dry matter extraction ratio was 29.6%-81.8%. The quality standard of methylophiopogonanone A transfer ratio was 4.86%-28.0%. The quality standard of methylophiopogonanone A yield was 4.75-20.1 μg·g^-1 pieces. The relative retention time of each characteristic peak and the reference peak was calculated by using the peak No. 4 methylophiopogonanone A as the reference peak. The relative retention time should be within ±10% of the specified value. CONCLUSION The methods are suitable in characteristic fingerprint analysis and content determination for Ophiopogon japonicus standard decoctions. The quality standards for Ophiopogon japonicus standard decoctions can be used for the quality evaluation of Ophiopogon japonicus formula granules.