Abstract: OBJECTIVE To analyze the literature metrology and visualization of the research of Ganoderma lucidum spore, to explore and summarize the research hotspots and development trends. METHODS Literatures related to Ganoderma lucidum spore powder were searched in CNKI, Pubmed and Web of Science databases, according to the different languages of the literature, it was divided into Chinese and English. CiteSpace was applied in the co-occurrence of country, institution, keywords, subject words and co-cited analysis, draw the corresponding knowledge map, the timeline view, and concluded the research hotspot and trend of Ganoderma lucidum spore powder. The main research hotspots, trend, clinical research and material basis of Ganoderma lucidum spore powder was summarized. RESULTS A total of 1 477 related papers were obtained, including 989 Chinese papers, 488 English papers, both showed a trend of rise, and the countries and organizations co-occurrence analysis showed that China and Chinese institutions as the main research unit, the research focus areas were pharmacological efficacy, which was mainly in anti-tumor, immune regulation, anti-epilepsy and other efficacy studies. Nearly five years emerging research focused on immune checkpoints, gut microbes. CONCLUSION Ganoderma lucidum spore powder has been widely studied in anti-tumor, immune regulation, anti-epilepsy, and has a good application prospect, but the research on material basis and quality control is relatively weak, and needs further study.