Abstract: OBJECTIVE To determine 4 flavonoid glycosides of epimedin A, epimedin B, epimedin C, and icariin in Bushen qiangshen capsules by HPLC, and investigate the transfer rate of icariin from Epimedii Folium. METHODS After ultrasonic extracted by 50% ethanol, the sample was separated on an Agilent Zorbax SB-C₁₈ (4.6 mm×250 mm, 5 μm) column with the gradient elution. The mobile phase consisted of acetonitrile and water, with a flow rate of 1.0 mL·min^–1. The column temperature was set at 40 ℃, and the wavelength was set at 270 nm. RESULTS good linear relationships of epimedin A, epimedin B, epimedin C and icariin were obtained with correlation coefficients≥0.999. The average recoveries were 104.2%, 103.2%, 105.3% and 103.7%, and RSDs were 1.4%, 1.9%, 0.9% and 1.6%, respectively. The content limit of icarii was determined ≥0.73 mg·g^–1, with the average transfer rate of icariin from 9 batches of self-made samples as 56.04%. Among 45 batches of samples from 11 manufacturers, 12 batches were below the limit, indicative of a rate of 26.7%. CONCLUSION The method has good reproducibility and strong specificity, which is simple and easy, and can be used to inspect the transfer rate of icarii in the preparation process and improve the quality control.