Abstract: OBJECTIVE To study the effect of polygonatum polysaccharide on glucose and lipid metabolism in type 2 diabetic rats and its mechanism.METHODS The type 2 diabetic rat model was constructed by injecting streptozotocin-sodium citrate solution to explore the effects of polygonatum polysaccharide on the physiological, blood and biochemical indicators of rats.The pathological changes of rat liver cells were observed by HE staining and oil red O staining.Western blotting was used to verify the mechanism of glucose and lipid metabolism in rats.RESULTS Polygonatum polysaccharide could significantly reduce alanine transaminase, aspartate transaminase, total cholesterol, triglyceride, low density lipoprotein cholesterol, creatinine, blood urea nitrogen, glycosylated hemoglobin and malondialdehyde, significantly increased their high density lipoprotein cholesterol, C-peptide, insulin, superoxide dismutase, catalase and glutathione peroxidase, and was able to improve the pathological degree of hepatocyte in rats.Western blotting results showed that polygonatum polysaccharide could upregulate the process of cell lipid metabolism through the PI3K/Akt pathway.CONCLUSION Polygonatum polysaccharide can protect the body from the pathological process induced by streptozotocin, and has a positive effect on the treatment of type 2 diabetes.