Abstract: OBJECTIVE To establish simultaneous determination of caryophylline, cineol, cinnamic aldehyde, linalool, α-pinene, β-pinene and borneol by gas chromatography and quantitative analysis of multi-components by single marker method, to control the quality of the 7 effective components in Meili Nianhua spray. METHODS HP-5 capillary column (30 m×0.32 mm, 0.25 μm) was used. The carrier gas was nitrogen. The temperature of hydrogen flame ionization detector was 240℃ and the injection temperature was 240℃. The injection volume was 1 μL and the shunt ratio was 20:1. RESULTS The calibration curves of caryophyllin, cineol, cinnamaldehyde, linalool, α-pinene, β-pinene and borneol were respectively in good linearity within the range of 0.020 0-0.140 0, 0.360 0-2.520 0, 0.205 0-1.435 0, 0.012 5-0.087 5, 0.070 0-0.490 0, 0.128 0-0.896 0, 0.060 0-0.420 0 mg·mL^-1(r ≥ 0.999 6), and the average contents were 0.120 5, 1.963 3, 0.650 4, 0.036 3, 0.791 9, 0.416 1 and 0.074 1 mg·mL^-1, respectively, with no significant difference compared with the internal standard method, and the corresponding average recoveries were 102.03%, 103.04%, 102.36%, 97.42%, 99.52%, 97.11% and 102.40%, RSDS were 1.38%, 0.61%, 0.72%, 1.32%, 1.37% and 0.99%, respectively. CONCLUSION The method is simple, accurate and reproducible, and can be used for the quality control of Meili Nianhua spray.