Abstract: OBJECTIVE To develop a method of quantitative analysis of multi-components by single-marker(QAMS) for simultaneous determination of six flavonoids in extract of Diospyros kaki Thunb. leaves. METHODS The Agilent Eclipse Plus C₁₈ column(250 mm×4.6 mm, 5 μm) was applied, the mobile phase was acetonitrile-0.1% phosphoric acid of gradient elution, the flow rate was 1.0 mL·min⁻¹, the detection wavelength was 360 nm, the column temperature was 30 ℃ and the injection volume was 10 mL. Used quercetin as a reference, the relative correction factors among the five flavonoids were detected by QAMS. The content of six index components in extract of Diospyros kaki Thunb. leaves. was determined by external standard method and QAMS. The results obtained from two different methods were compared, verified the practicability and stability of QAMS. RESULTS The QAMS was established and used to determine the six flavonoids in the extract of Diospyros kaki Thunb. leaves. The results showed that the relative correction factors had good system applicability and reproducibility, and there was no significant difference between method QAMS and external standard method. CONCLUSION The established QAMS method for simultaneous determination of six flavonoids in extract of Diospyros kaki Thunb. leaves is simple, accurate, reproducible and feasible to quantify multiple components in extract of Diospyros kaki Thunb. leaves.