Abstract: OBJECTIVE To select anti-hepatocarcinoma effective parts of Akebiae Fructus. METHODS The H22 subcutaneously transplanted tumor in mice was applied to screen the anti-hepatocarcinoma active fractions from the ethanol extract, petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract and the water extract from residue extracted from ethanol, the dosage was crude 20 g drug·kg^‒1; the anti-hepatocarcinoma effect of ethanol extract on the nude mice model of human hepatoma line BEL-7404 cell subcutaneously transplanted tumor was studied. RESULTS The results showed that the ethanol extract and n-butanol extract of Akebiae Fructus had obvious anti-tumor effect on H22 mice model of subcutaneous transplantation of liver cancer, the inhibition rates were 46.87% and 45.31%, respectively. The other parts had no obvious antitumor effect; the high-dose group(crude drug 20 g·kg^‒1) and the medium dose group(crude drug 10 g·kg^‒1) of the ethanol extract of Akebiae Fructus had obvious antitumor effect on BEL-7404 transplanted tumor model in nude mice, and the inhibition rates were 57.5% and 40.0%, respectively. CONCLUSION The ethanol extract and its n-butanol extract are the antitumor active fractions of Akebiae Fructus.