Abstract: OBJECTIVE To determine content of 5 flavanones of 3,3',5,7- tetrahydroxy-4'-methoxyflavanone, 3',5,5',7- tetrahydroxy-flavanone, 3,3',4',5-tetrahydroxy-7-methoxyflavanone, 3,3',5-trihydroxy-4',7-dimethoxyflavanone, 5-blumeatin in Blumea balsamifera. METHODS The chromatographic separation was achieved on AKZONOBEL Kromasil 100-5C₁₈ (4.6 mm×250 mm, 5 µm) column with the mobile phase consisting of acetonitrile(A)-0.2% HAc(B) by gradient elution program(0-10 min, 20%A; 10-15 min, 20%→25%A; 15-25 min, 25%A; 25-50 min, 25%→65%A) at the flow rate of 1.0 mL·min^-1, detection wavelength was set at 289 nm, and the column temperature was 35℃, and the injection volume was 10 μL. RESULTS Linearity relationships of 5 flavanones were good, and the average recoveries were from 98.73% to 101.84%. CONCLUSION The method is simple, accurate and repeatable and it is suitable for quality control of Blumea balsamifera.