Abstract: OBJECTIVE To study the inhibition effect and mechanism of dl-3-n-butylphthalidle(NBP) on the inflammatory response of astrocytes(Ast) induced by lipopolysaccharide(LPS) and its effect on the expression of connexin 43(Cx43). METHODS Ast was treated with gradient concentration of NBP, and 1 μg·mL^-1 LPS induced inflammatory response. Lactate dehydrogenase(LDH) leakage was detected by kit, cell survival was detected by CCK-8, NO release was detected by Gries, IL-1β, IL-6 and TNF-α expression were detected by ELISA. Western blotting was used to detect the expression of p65, p-IκB and Cx43. The distribution of Cx43 was detected by immunofluorescence staining. SiRNA silenced the expression of Cx43 in Ast. After LPS induced inflammation, the release of inflammatory factors and cell survival were measured. RESULTS NBP could reduce LDH leakage, improve cell survival rate, inhibit the release of inflammatory factors, and inhibit the expression of p65 and p-IκB in Ast cells. NBP also inhibited the expression of Cx43 in Ast. After silencing Cx43 with siRNA, the inflammatory response of Ast was inhibited. CONCLUSION NBP can inhibit LPS induced Ast inflammatory response, and Cx43 is related to the occurrence of inflammatory response. The effect of NBP on Ast is related to the inhibition of Cx43 expression.