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    UPLC同时测定生脉饮口服液中五味子醇甲、五味子醇乙、五味子甲素的含量

    Simultaneous Determination of Schisandrin A, Schisandrin B and Deoxyschizandrin in Shengmaiyin Oral Liquid by UPLC

      摘要
    • 摘要: 目的 建立UPLC测定生脉饮口服液中有效成分（五味子醇甲、五味子醇乙、五味子甲素）含量的方法。方法 采用XBridge® BEH C18色谱柱（2.1 mm×100 mm，2.5 μm），以乙腈（A）-水（B）为流动相进行梯度洗脱，流速0.5 mL·min-1，进样量5 μL；柱温为40℃；检测波长为203 nm。结果 五味子醇甲、五味子醇乙、五味子甲素检测质量浓度线性范围分别为1.950~124.8μg·mL-1（r=0.999 9）、1.375~88.00μg·mL-1（r=0.999 9）、1.594~102.0μg·mL-1（r=0.999 9）；定量限≤0.137 5μg·mL-1，检测限≤0.068 8μg·mL-1；仪器精密度、稳定性、重复性试验的RSD≤1.18%；加样回收率分别为98.65%（RSD=0.82%），98.47%（RSD=1.21%），99.24%（RSD=0.99%）。结论 该方法操作简便，仪器精密度、稳定性、重复性好，可用于生脉饮口服液中五味子醇甲、五味子醇乙、五味子甲素含量测定。

       

      Abstract: OBJECTIVE To establish quantitative analysis method of effecctive components(schisandrin A, schisandrin B and deoxyschizandrin) in Shengmaiyin oral liquid by UPLC. METHODS The samples were separated on a XBridge® BEH C18 (2.1 mm×100 mm, 2.5 μm). The mobile phase was composed of acetonitrile(A) and water(B) with gradient elution, the flow rate was 0.5 mL·min-1 and the injection volume was 5 μL. The column temperature was 40℃ and detection wavelength was 203 nm. RESULTS The linear ranges were 1.950-124.8 µg·mL-1 for schisandrin A(r=0.999 9), 1.375-88.00 µg·mL-1 for schisandrin B(r=0.999 9), 1.594-102.0 µg·mL-1 for deoxyschizandrin(r=0.999 9). The limits of quantify were no more than 0.137 5 µg·mL-1, limits of detection were no more than 0.068 8 µg·mL-1; RSDs of instrument precision, stability and reproducibility tests were ≤ 1.18%; the recoveries were 98.65%(RSD=0.82%), 98.47%(RSD=1.21%), 99.24%(RSD=0.99%), respectively. CONCLUSION The method is simple, precise, stable and reproducible, and can be used for simultaneous determination of schisandrin A, schisandrin B and deoxyschizandrin in Shengmaiyin oral liquid.

       

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