Abstract: OBJECTIVE To explore the characteristics of various detection methods through the test of Salmonella in proficiency testing samples, so as to provide reference for the rapid detection and identification of Salmonella in food and medicine. METHODS According to the operation instruction for proficiency testing issued by National Institutes for Food and Drug Control, combined with the national food safety standard GB4789.4-2016 and the common detection methods of Salmonella in authors' laboratory, the samples were isolated and identified. RESULTS The two samples were identified as Salmonella typhimurium and Salmonella Ⅲb(Salmonella enterica ssp. diarizonae). CONCLUSION Based on the traditional methods of GB and ChP, other simple and rapid detection methods are added to ensure the rapid acquisition and accuracy of the results.